California: Although the mechanism remains unclear, high salt concentrations may inactivate viruses by damaging the viral nucleic acid. With high contents of organic macromolecules, phase-separated particles with organic- and inorganic-rich domains or amorphous particles containing trapped moisture may form, potentially enhancing viral and bacterial survival. Furthermore, the pH of aerosol particles is RH, size, and composition dependent, and the pH of aerosol droplet surfaces may be different from the droplet bulk. Indeed, predicting the evolving aerosol pH is challenging, particularly when the facile partitioning of water-soluble acidic and basic components from the ambient environment is considered, even before the influence of aerosol pH on microorganism survival is considered.
Laboratory strategies to assess the airborne stability of a pathogen must either be capable of simulating every aspect of the real-world environment in which transmission occurs or sufficient control over the conditions must be achieved such that the influence of individual processes and properties on survival can be assessed independently. Goldberg rotating drums have been widely used over many decades to assess airborne pathogen stability and have been used to investigate the airborne survival of SARS-CoV-2. More specifically, studies have examined the dependence of infectivity on time (20 min to 16 h), RH (40 to 70 per cent), and the presence of UVC light with measurements in aerosols composed of cell culture media (Dulbecco's modified Eagle medium [DMEM] and minimal essential media [MEM]) and artificial saliva. All studies concentrate on equilibrated particle sizes of ~5 um (mass median aerodynamic diameter). A nebulizer is used to generate a cloud of aerosolized pathogen that is suspended by the rotation of the drum. The initial environmental conditions within the drum can be controlled by mixing the output of the nebulizer with a flow of humidity- and temperature-controlled air. However, operation with stable environmental conditions can be challenging; for example, as the droplets evaporate and equilibrate to the set humidity, the water they release can cause the humidity within the drum to increase. In addition, dynamic changes in liquid water content within the freshly nebulized aerosol-cloud do not replicate the very rapid changes that can accompany the extremely low concentrations of the exhaled aerosol. This precludes any study of short-term decreases in pathogen viability that may be critical to understanding close contact transmission and the immediate consequences of exhalation on microbe survival.